Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | NRG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NRG1, ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, NDF, SMDF, MST131 |
| Product description | Neuregulin 1 (NRG1) interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.1) Masuda, K., PLoS Med. 5 (5), E94 (2008). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NRG1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NRG1 antibody can be used for detection of NRG1 by ELISA at 1:62500. NRG1 antibody can be used for detection of NRG1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NRG1 |
|---|---|
| Alternative Names | NRG1 ARIA GGF GGF2 HGL HRG HRG1 HRGA NDF SMDF MST131 |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NRG1 antibody can be used for detection of NRG1 by ELISA at 1:62500. NRG1 antibody can be used for detection of NRG1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NRG1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
