Application
Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.Application
Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 μg of His antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.| Product Name | His-tag Antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | His-tag, His, 6xHis |
| Product description | Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. His-tag is one of the more popular epitope tags for expressed recombinant proteins. |
| Immunogen | His-tag antibody was raised against a synthetic peptide containing HHHHHH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.: |
|
| Species Reactivity | Tag |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | N/A |
|---|---|
| Alternative Names | His-tag His 6xHis |
| Molecular Weight(MW) | N/A |
| Tissue Specificity | His-tag antibody will detect amino- or carboxy-terminal His-tagged recombinant proteins. |
Application
Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.
Application
Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 μg of His antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.| Positive Control | 1) N/A |
|---|---|
| Application Notes | His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | His-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | His-tag antibody is supplied in PBS containing 0.02% sodium azide. |
