Application
Western blot analysis of HSD3B1 using rabbit polyclonal HSD3B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.| Product Name | HSD3B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Trophoblast antigen FDO161G, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxy-5-ene steroid dehyd |
| Product description | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.1) Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010) |
| Immunogen | This HSD3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human HSD3B1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSD3B1 |
|---|---|
| Alternative Names | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I 3-beta-HSD I Trophoblast antigen FDO161G 3-beta-hydroxy-Delta(5)-steroid dehydrogenase 3-beta-hydroxy-5-ene steroid dehyd |
| Molecular Weight(MW) | 42 kDa |
Application
Western blot analysis of HSD3B1 using rabbit polyclonal HSD3B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
