HSD3B1, HSD3B, HSDB3, I, HSDB3A, SDR11E1, 3BETAHSD
Product description
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta (5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.1) Ross, R.W., (2008) J. Clin. Oncol. 26 (6), 842-847.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSD3B1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HSD3B1 antibody can be used for detection of HSD3B1 by ELISA at 1:62500. HSD3B1 antibody can be used for detection of HSD3B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Monkey brain extract at: 1:1000.
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
HSD3B1 antibody can be used for detection of HSD3B1 by ELISA at 1:62500. HSD3B1 antibody can be used for detection of HSD3B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HSD3B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.