WB
Western blot analysis of various lysates using HSD3B1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IF-P
Immunofluorescence analysis of paraffin embedded rat adrenal gland using HSD3B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IF-P
Immunofluorescence analysis of paraffin embedded human placenta using HSD3B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IF-P
Immunofluorescence analysis of paraffin embedded mouse adrenal gland using HSD3B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | HSD3B1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human HSD3B1 (NP_000853.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSD3B1 |
|---|---|
| Gene Synonyms | HSD3B HSDB3 HSDB3A SDR11E1 3BETAHSD |
| Gene Full Name | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 |
| Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016] |
| Alternative Names | HSD3B HSDB3 HSDB3A SDR11E1 3BETAHSD HSD3B1 |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Mitochondrion membrane, Single-pass membrane protein. |
WB
Western blot analysis of various lysates using HSD3B1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IF-P
Immunofluorescence analysis of paraffin embedded rat adrenal gland using HSD3B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IF-P
Immunofluorescence analysis of paraffin embedded human placenta using HSD3B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IF-P
Immunofluorescence analysis of paraffin embedded mouse adrenal gland using HSD3B1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% Sodium azide, 50% glycerol, pH7.3. |
