WB
Western blot analysis of HSD3B1 on human placenta tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSD3B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of HSD3B1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Product Name | HSD3B1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human HSD3B1 aa 320-373. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:100-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSD3B1 |
|---|---|
| Gene Synonyms | HSD3B HSDB3 HSDB3A SDR11E1 3BETAHSD |
| Gene Full Name | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 |
| Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016] |
| Alternative Names | 3 beta and steroid delta isomerase 1 antibody 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody 3 beta HSD I antibody 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody 3-beta-HSD I antibody 3-beta-hydroxy-5-ene steroid dehydrogenase antibody 3BETAHSD antibody 3BH antibody 3BHS1_HUMAN antibody 3BHSD antibody 3 beta and steroid delta isomerase 1 antibody 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody 3 beta HSD I antibody 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody 3-beta-HSD I antibody 3-beta-hydroxy-5-ene steroid dehydrogenase antibody 3BETAHSD antibody 3BH antibody 3BHS1_HUMAN antibody 3BHSD antibody Delta-5-3-ketosteroid isomerase antibody HGNC:5217 antibody HSD3B antibody HSD3B1 antibody HSDB3 antibody HSDB3A antibody Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 antibody Hydroxy delta 5 steroid dehydrogenase antibody Progesterone reductase antibody SDR11E1 antibody Steroid Delta-isomerase antibody trophoblast antigen antibody Trophoblast antigen FDO161G antibody |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Endoplasmic reticulum, Membrane, Mitochondrion. |
WB
Western blot analysis of HSD3B1 on human placenta tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSD3B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of HSD3B1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:2000-1:5000 IHC:1:100-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
