Application
Immunohistochemistry staining of ICAM1 in spleen tissue using ICAM1 monoclonal Antibody.| Product Name | ICAM1 Antibody, Monoclonal |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58 |
| Immunogen | ICAM1 monoclonal antibody was raised against Human monocytes (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIP |
| ICAM1 antibody can be used in Western Blot starting at 0.5 - 1 μg/mL, and immunohistochemistry starting at 5 μg/mL. Flow Cytometry 1:50-1:100 Immunohistochemistry (frozen)- 1:50-1:100, Immunohistochemistry (FFPE), Immunoprecipitation. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry Recommended positive control tissue: Human Tonsil. Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function. Removal of sodium azide is recommended prior to use in functional assays.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ICAM1 |
|---|---|
| Alternative Names | ICAM1 BB2 CD54 antigen CD54 ICAM-1 p3.58 |
| Tissue Specificity | Species Crossreactivity: Clone 3H1547 is reported to crossreact with porcine endothelium. |
Application
Immunohistochemistry staining of ICAM1 in spleen tissue using ICAM1 monoclonal Antibody.| Application Notes | ICAM1 antibody can be used in Western Blot starting at 0.5 - 1 μg/mL, and immunohistochemistry starting at 5 μg/mL. Flow Cytometry 1:50-1:100 Immunohistochemistry (frozen)- 1:50-1:100, Immunohistochemistry (FFPE), Immunoprecipitation. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry Recommended positive control tissue: Human Tonsil. Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function. Removal of sodium azide is recommended prior to use in functional assays.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store ICAM1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, pH 7.4, 0.09% sodium azide |
