Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R (Ab-1161). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R (Ab-1161).Application
Western blot analysis of lysed extracts from 293 cells using IGF-1R (Ab-1161).| Product Name | IGF 1R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IGFR, CD221, IGFIR, JTK13, MACS, PRKCSL, Insulin-like growth factor I receptor, IGF1R, kinase IGF1R |
| Product description | This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.1) Li S, et al. (1994) J Biol Chem; 269 (51) . Hernandez-Sanchez C, et al. (1995) J Biol Chem. |
| Immunogen | IGF-1R (Ab-1161) antibody was raised against a peptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from Human IGF-1R . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF1R |
|---|---|
| Alternative Names | IGFR CD221 IGFIR JTK13 MACS PRKCSL Insulin-like growth factor I receptor IGF1R kinase IGF1R |
| Molecular Weight(MW) | 200 95 kDa |
| Tissue Specificity | This antibody detects endogenous level of total IGF-1R protein. |
| Entrez Gene | 3480 |
|---|---|
| Protein Accession | NP_000866.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R (Ab-1161). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R (Ab-1161).
Application
Western blot analysis of lysed extracts from 293 cells using IGF-1R (Ab-1161).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
