Application
Immunohistochemical analysis of paraffin - embedded human gastric adenocarcinoma (top) and lung squamous cell carcinoma (bottom) showing membrane location using IGF1R antibody with DAB staining.Application
Western blot analysis using IGF1R monoclonal antibody against truncated IGF1R - His recombinant protein.| Product Name | IGF1R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-like growth factor I receptor, IGF-I receptor, IGFR, CD221, IGFIR, JTK13 |
| Product description | IGF1R(insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.1) Zhu Z. Jiang W. Thompson HJ. Carcinogenesis. 2003, Jul, 24(7):1225-31. Epub 2003 May 9. |
| Immunogen | Ni-NTA purified truncated recombinant IGF1R-His expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IGF1R |
|---|---|
| Alternative Names | Insulin-like growth factor I receptor IGF-I receptor IGFR CD221 IGFIR JTK13 |
Application
Immunohistochemical analysis of paraffin - embedded human gastric adenocarcinoma (top) and lung squamous cell carcinoma (bottom) showing membrane location using IGF1R antibody with DAB staining.
Application
Western blot analysis using IGF1R monoclonal antibody against truncated IGF1R - His recombinant protein.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IGF1R monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
