Application
Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry.Application
Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35ug/lane)| Product Name | IGF1R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain, IGF1R |
| Product description | The IGF1R receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.1) Song, R.X., et al., Proc. Natl. Acad. Sci. U.S.A. 101(7):2076-2081 (2004). |
| Immunogen | This IGF1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human IGF1R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IGF1R |
|---|---|
| Alternative Names | Insulin-like growth factor 1 receptor Insulin-like growth factor I receptor IGF-I receptor CD221 Insulin-like growth factor 1 receptor alpha chain Insulin-like growth factor 1 receptor beta chain IGF1R |
| Molecular Weight(MW) | 155 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry.
Application
Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
