Application
Western blot analysis of extract from 293 cells using IGF-1R (Phospho-Tyr1280).| Product Name | IGF 1R (phospho Tyr1280) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IGFR, CD221, IGFIR, JTK13, P53, IGFR |
| Product description | This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. Ref.19 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, Ref.21 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.1) Adams, T. E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093Baserga, R. et al. (2000) Oncogene 19, 5574-5581Scheidegger, K. J. et al. (2000) J. Biol. Chem. 275, 38921-38928. O'Connor R, et al. Mol Cell Biol 1997 Jan; 17 (1): 427-35 |
| Immunogen | IGF-1R (Phospho-Tyr1280) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1280 (S-F-Y (p) -Y-S) derived from Human IGF-1R. |
| Modification | p-Tyr1280 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF1R |
|---|---|
| Alternative Names | IGFR CD221 IGFIR JTK13 P53 IGFR |
| Molecular Weight(MW) | 200, 95 kDa |
| Tissue Specificity | This antibody detects endogenous levels of IGF-1R only when phosphorylated at tyrosine 1280. |
| Entrez Gene | 3480 |
|---|---|
| Protein Accession | NP_000866.1 |
Application
Western blot analysis of extract from 293 cells using IGF-1R (Phospho-Tyr1280).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
