Application
Western blot analysis using IgG monoclonal antibody against human serum IgG.| Product Name | IgG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD32, FCG2, FCGR2A1, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, IgG Fc receptor II-a, FcGR, CD32A, FCGR2 |
| Product description | Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.1) Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition. |
| Immunogen | Chrompure Human IgG, Fc Fragment. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FCGR2A |
|---|---|
| Alternative Names | CD32 FCG2 FCGR2A1 IGFR2 Low affinity immunoglobulin gamma Fc region receptor II-a CDw32 IgG Fc receptor II-a FcGR CD32A FCGR2 |
Application
Western blot analysis using IgG monoclonal antibody against human serum IgG.| Application Notes | Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IgG monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
