Application
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentratApplication
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentratApplication
To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-6 (38-200) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.| Product Name | IL-6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-6 (human Interleukin-6). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,Neut,WB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (1.0 ng/mL), a concentration of 0.2 -0.4 μg/mL of this antibody is required. ELISA: To detect hIL-6 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich: To detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-6 (XP-5192Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL6 |
|---|---|
| Alternative Names | HGF HSF BSF2 IL-6 IFNB2 Interleukin-6 B-cell stimulatory factor 2 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentrat
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentrat
Application
To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-6 (38-200) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (1.0 ng/mL), a concentration of 0.2 -0.4 μg/mL of this antibody is required. ELISA: To detect hIL-6 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich: To detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-6 (XP-5192Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Western Blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-6 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
