Interleukine 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts and epithelial cells. It has a wide variety of biological functions through activating a cellsurface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. The cytokine plays an essential role in the final differentiation of b-cells into Igsecreting cells, and induces myeloma, plasmacytoma growth, nerve cells differentiations. In addition, IL-6 is an important regulator of acute phase reaction.1) Ota, N. et al. 2001. J. Hum. Genet. 46: 267-272.
Immunogen
Ni-NTA purified recombinant human IL-6 expressed in E. Coli strain BL21 (DE3).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ELISAWB
Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test.:
Western blot analysis using IL6 monoclonal antibody against truncated IL6 recombinant protein.
Application Notes
Western Blot:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
IL-6 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.