Application
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue sApplication
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.Application
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.| Product Name | IL-6 Antibody [1.2-2B11-2G10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. |
| Immunogen | The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-6 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISA,WB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IL-6 when used with our biotinylated antigen affinity purified anti-human IL-6 (XP-5193Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.25-0.50 ng/lane, under reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IL6 |
|---|---|
| Alternative Names | Interleukin 6 IFNB2 Interleukin-6 B-cell stimulatory factor 2 IL-6 HGF HSF BSF2 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue s
Application
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Application
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IL-6 when used with our biotinylated antigen affinity purified anti-human IL-6 (XP-5193Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.25-0.50 ng/lane, under reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-6 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
