JMJD3 is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.1) Hong, S., (2007) Proc. Natl. Acad. Sci. U.S.A. 104 (47), 18439-18444.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human JMJD3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
JMJD3 antibody can be used for detection of JMJD3 by ELISA at 1:312500. JMJD3 antibody can be used for detection of JMJD3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
JMJD3 antibody can be used for detection of JMJD3 by ELISA at 1:312500. JMJD3 antibody can be used for detection of JMJD3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store JMJD3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.