Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | JMJD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JMJD3, KIAA0346, JMJD3 |
| Product description | JMJD3 is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human JMJD3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| JMJD3 antibody can be used for detection of JMJD3 by ELISA at 1:1562500. JMJD3 antibody can be used for detection of JMJD3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KDM6B |
|---|---|
| Alternative Names | JMJD3 KIAA0346 JMJD3 |
| Molecular Weight(MW) | 70 kDa |
Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | JMJD3 antibody can be used for detection of JMJD3 by ELISA at 1:1562500. JMJD3 antibody can be used for detection of JMJD3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store JMJD3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
