Application
Western blot analysis of extract from K-562, HeLa, JK and 293 cells using LC3 Antibody.| Product Name | LC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3 |
| Product description | Probably involved in formation of autophagosomal vacuoles (autophagosomes).1) He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J. O., Yu L. J. Biol. Chem. 278:29278-29287 (2003) Tanida I., Sou Y. -S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E. J. Biol. Chem. 279:36268-36276 (2004) |
| Immunogen | LC3 antibody was raised against a peptide sequence around aa. 112-116 (V-Y-A-S-Q) derived from Human LC3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP1LC3A |
|---|---|
| Alternative Names | LC3 LC3A ATG8E MAP1ALC3 MAP1BLC3 |
| Molecular Weight(MW) | 19 kDa |
| Tissue Specificity | This antibody detects endogenous level of total LC3 protein. |
| Entrez Gene | 84557 |
|---|---|
| Protein Accession | NP_115903.1 |
Application
Western blot analysis of extract from K-562, HeLa, JK and 293 cells using LC3 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
