Application
Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody.Application
Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody #21402.| Product Name | LC3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, Autophagy-related protein LC3 A |
| Product description | Probably involved in formation of autophagosomal vacuoles (autophagosomes).1) Hammarback JA, et al. Neuron 7 (1): 129-39, 1991. He H, et al. J. Biol Chem 278 (31): 29278-87, 2003. |
| Immunogen | LC3A antibody was raised against a peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP1LC3A |
|---|---|
| Alternative Names | LC3 LC3A ATG8E MAP1ALC3 MAP1BLC3 Microtubule-associated protein 1 light chain 3 alpha MAP1A/1B light chain 3 A MAP1A/MAP1B LC3 A MAP1 light chain 3-like protein 1 Autophagy-related protein LC3 A |
| Molecular Weight(MW) | 18, 16 kDa |
| Tissue Specificity | This antibody detects endogenous level of total LC3A protein. |
| Entrez Gene | 84557 |
|---|---|
| Protein Accession | NP_115903.1 |
Application
Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody.
Application
Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody #21402.| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
