Application
Immunohistochemistry staining of LEFTY2 in uterus, myometrium tissue using LEFTY2 monoclonal Antibody.| Product Name | LEFTY2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4 |
| Product description | During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-β family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-β (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-β actions are directed by ligand-induced activation of TGF-β receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-β superfamily, inhibits TGF-β signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-β receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-β-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-β superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 kDa) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity. |
| Immunogen | LEFTY2 monoclonal antibody was raised against recombinant LEFTY2 (Human). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LEFTY2 |
|---|---|
| Alternative Names | LEFTY2 EBAF LEFTYA Protein lefty-2 LEFTA TGFB4 Protein lefty-A TGF-beta-4 |
| Tissue Specificity | A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY. |
Application
Immunohistochemistry staining of LEFTY2 in uterus, myometrium tissue using LEFTY2 monoclonal Antibody.| Application Notes | LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store LEFTY2 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
