Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | MAP2K3 (phospho Thr222) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.1) Derijard B., Science 267:682-685(1995). |
| Immunogen | MAP2K3 (phospho Thr222) antibody was raised against a peptide sequence around phosphorylation site of threonine 222 (A-K-T (p)-M-D) derived from Human MAP2K3 . |
| Modification | p-Thr222 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K3 |
|---|---|
| Alternative Names | MAP2K3 MAPKK 3 MEK3 MP2K3 PRKMK3 |
| Molecular Weight(MW) | 39 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222. |
| Entrez Gene | 5606 |
|---|---|
| Protein Accession | NP_659731.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
