Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Ab-189). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Ab-189).Application
Western blot analysis of lysed extracts from MDA cells using MKK3 (Ab-189).| Product Name | MKK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, ERK1, PRKM3, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3 |
| Product description | MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.1) Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55. |
| Immunogen | MKK3 (Ab-189) antibody was raised against a peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K3 |
|---|---|
| Alternative Names | MEK3 MKK3 MAPKK3 PRKMK3 SAPKK2 SAPKK-2 ERK1 PRKM3 MAP kinase kinase 3 MAP2K3 MAPK/ERK kinase 3 MAPKK 3 MEK3 |
| Molecular Weight(MW) | 40 kDa |
| Tissue Specificity | This antibody detects endogenous level of total MKK3 protein. |
| Entrez Gene | 5606 |
|---|---|
| Protein Accession | NP_002747.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Ab-189). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Ab-189).
Application
Western blot analysis of lysed extracts from MDA cells using MKK3 (Ab-189).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
