Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).| Product Name | MKK3 (phospho Ser189) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3 |
| Product description | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.1) Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55. |
| Immunogen | MKK3 (Phospho-Ser189) antibody was raised against a peptide sequence around phosphorylation site of serine 189 (V-D-S (p) -V-A) derived from Human MKK3. |
| Modification | p-Ser189 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K3 |
|---|---|
| Alternative Names | MEK3 MKK3 MAPKK3 PRKMK3 SAPKK2 SAPKK-2 MAP kinase kinase 3 MAP2K3 MAPK/ERK kinase 3 MAPKK 3 MEK3 |
| Molecular Weight(MW) | 40 kDa |
| Tissue Specificity | This antibody detects endogenous level of MKK3 only when phosphorylated at serine189. |
| Entrez Gene | 5606 |
|---|---|
| Protein Accession | NP_002747.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
