Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | MAP3K8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP3K8, COT, EST, ESTF, TPL2, MEKK8, Tpl-2, c-COT |
| Product description | MAP3K8 is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.1) Jia, Y., et al., (2005) Arch. Biochem. Biophys. 441 (1), 64-74. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAP3K8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MAP3K8 antibody can be used for detection of MAP3K8 by ELISA at 1:1562500. MAP3K8 antibody can be used for detection of MAP3K8 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP3K8 |
|---|---|
| Alternative Names | MAP3K8 COT EST ESTF TPL2 MEKK8 Tpl-2 c-COT |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MAP3K8 antibody can be used for detection of MAP3K8 by ELISA at 1:1562500. MAP3K8 antibody can be used for detection of MAP3K8 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MAP3K8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
