Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MCK10 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MCK10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain.1) Vogel, W., et al., Mol. Cell 1(1):13-23 (1997). |
| Immunogen | This MCK10 antibody is generated from rabbits immunized with recombinant human MCK10 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | DDR1 |
|---|---|
| Alternative Names | Epithelial discoidin domain-containing receptor 1 Epithelial discoidin domain receptor 1 CD167 antigen-like family member A Cell adhesion kinase Discoidin receptor tyrosine kinase HGK2 Mammary carcinoma kinase 10 MCK-10 Protein-tyrosine kinase 3A |
| Molecular Weight(MW) | 101 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MCK10 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
