Application
Confocal immunofluorescent analysis of MDK Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
Confocal immunofluorescent analysis of MDK Antibody with Hela cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
MDK Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).| Product Name | MDK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004 [PubMed 15197188]).1) Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010) Kerzerho, J., et al. J. Immunol. 185(1):418-423(2010) Yao, X., et al. Acta Pharmacol. Sin. 31(5):629-637(2010) Zhang, Z.H., et al. Cell Prolif. 43(2):184-194(2010) Rice, G.E., et al. J. Exp. Clin. Cancer Res. 29, 62 (2010) : |
| Immunogen | This MDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the C-terminal region of human MDK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MDK |
|---|---|
| Alternative Names | Midkine MK Amphiregulin-associated protein ARAP Midgestation and kidney protein Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein MDK MK1 NEGF2 |
| Molecular Weight(MW) | 16 kDa |
Application
Confocal immunofluorescent analysis of MDK Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
Confocal immunofluorescent analysis of MDK Antibody with Hela cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
MDK Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
