WB
COLO 320 cells were subjected to SDS PAGE followed by western blot with Midkine antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with Midkine antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
WB result of Midkine antibody (1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Midkine transfected SH-SY5Y cells.IHC
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using Midkine antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using Midkine antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-Midkine (IP: 4ug; Detection:1:3000) with mouse embryo tissue lysate 4800 ug.ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Midkine antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Midkine Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MDK protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDK |
|---|---|
| Gene Synonyms | MK ARAP NEGF2 |
| Gene Full Name | midkine |
| Gene Summary | This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012] |
| Alternative Names | MDK Amphiregulin-associated protein Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein |
| Molecular Weight(MW) | 16 kDa |
| Cellular Localization | Secreted |
WB
COLO 320 cells were subjected to SDS PAGE followed by western blot with Midkine antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with Midkine antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
WB result of Midkine antibody (1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Midkine transfected SH-SY5Y cells.
IHC
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using Midkine antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using Midkine antibody at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-Midkine (IP: 4ug; Detection:1:3000) with mouse embryo tissue lysate 4800 ug.
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Midkine antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
