Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 (phospho-Ser166).| Product Name | MDM2 (phospho Ser166) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues1) Haupt, Y. et al. (1997) Nature 387, 296-299. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. Mayo, L. D. and Donner, D. B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. |
| Immunogen | MDM2 (phospho-Ser166) antibody was raised against a peptide sequence around phosphorylation site of Serine 166 (A-I-S (p) -E-T) derived from Human MDM2. |
| Modification | p-Ser166 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDM2 |
|---|---|
| Alternative Names | HDMX hdm2 ACTFS ESA1 M17S1 |
| Molecular Weight(MW) | 90 kDa |
| Tissue Specificity | This antibody detects endogenous level of MDM2 only when phosphorylated at serine 166. |
| Entrez Gene | 4193 |
|---|---|
| Protein Accession | NP_002383.2 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 (phospho-Ser166).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
