Application
Western blot analysis of lysed extracts from 293 and HeLa cells using MDM2 Antibody.| Product Name | MDM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .1) Haupt, Y. et al. (1997) Nature 387, 296-299. Mayo, L. D. and Donner, D. B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. |
| Immunogen | MDM2 antibody was raised against a peptide sequence around aa.18~22 (Q-I-P-A-S) derived from Human MDM2. . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDM2 |
|---|---|
| Alternative Names | HDMX hdm2 ACTFS CCN5 CT58 CTGFL UNQ228/PRO261 HDMX |
| Molecular Weight(MW) | 90 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total MDM2 protein. |
| Entrez Gene | 4193 |
|---|---|
| Protein Accession | NP_002383.2 |
Application
Western blot analysis of lysed extracts from 293 and HeLa cells using MDM2 Antibody.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
