Application
Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 (Phospho-Ser186/Ser188) Antibody.| Product Name | MDM2 (phospho Ser186/Ser188) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues1) M Zhou, Blood, Mar 1995; 85: 1608 - 1614. |
| Immunogen | MDM2 (phospho Ser186/Ser188) antibody was raised against a peptide sequence around phosphorylation site of Ser186/Ser188 (H-K-S (p)-D-S (p)-I-S) derived from Human MDM2. |
| Modification | p-Ser186/Ser188 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MDM2 |
|---|---|
| Alternative Names | Oncoprotein Mdm2 p53-binding protein Mdm2 Ubiquitin-protein ligase E3 Mdm2 |
| Molecular Weight(MW) | 55 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188. |
| Entrez Gene | 4193 |
|---|---|
| Protein Accession | NP_001138811.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 (Phospho-Ser186/Ser188) Antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
