Application
Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).| Product Name | MEK5 (phospho Ser311 / Thr315) Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).1) Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 270(21):12665-9. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5. |
| Modification | p-Ser311 / Thr315 |
| Clonality | Polyclonal |
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| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, chicken, bovine, canine, non-human primate, Xenopus, and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. | |
| Species Reactivity | BovineDogHumanMouseRatXenopusZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Map2k5 |
|---|---|
| Alternative Names | Mek5 Mek5 Mkk5 Prkmk5 MAP kinase kinase 5 |
| Molecular Weight(MW) | 49 |
Application
Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, chicken, bovine, canine, non-human primate, Xenopus, and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. |
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| Form | Liquid |
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| Storage Instructions | MEK5 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
