Application
Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody.| Product Name | MEK5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | A family of protein kinases located upstream of the MAP kinases andresponsible for their activation has been identified. The prototype member ofthis family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present inthe Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to acti-vate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activatesboth p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway.1) Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891. Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926. Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442. Holland, P. M., et al. 1997. J. Biol. Chem. 272: 24994-24998. |
| Immunogen | MEK5 antibody was raised against a peptide sequence around aa. 311-315 (K-T-Y-V-G ) derived from Rat MEK5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Map2k5 |
|---|---|
| Alternative Names | Mek5 MAP kinase kinase 5 Map2k5 MAPKK 5 MAPK/ERK kinase 5 |
| Molecular Weight(MW) | 49 kDa |
| Tissue Specificity | This antibody detects endogenous level of total MEK5 protein. |
| Entrez Gene | 29568 |
|---|---|
| Protein Accession | NP_001029159.1 |
Application
Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody.| Application Notes | Western Blot: 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
