Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
Western blot analysis of Map2k5 using rabbit polyclonal Mouse Map2k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k5 gene.| Product Name | Map2k5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5 |
| Product description | Map2k5 acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.1) Spiering, D., et al. J. Biol. Chem. 284(37):24972-24980(2009) |
| Immunogen | This Mouse Map2k5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the C-terminal region of mouse Map2k5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Map2k5 |
|---|---|
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 5 MAP kinase kinase 5 MAPKK 5 MAPK/ERK kinase 5 MEK 5 Map2k5 Mek5 Mkk5 Prkmk5 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
Western blot analysis of Map2k5 using rabbit polyclonal Mouse Map2k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k5 gene.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
