Application
Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 10 μg/mL.Application
Immunohistochemistry of Nephrin in rat kidney tissue with Nephrin antibody at 10 μg/mL.Application
Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 5 μg/mL.Application
Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.| Product Name | Nephrin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor |
| Product description | Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.1) Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein - Nephrin - is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582. |
| Immunogen | Nephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC-p:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPHS1 |
|---|---|
| Alternative Names | Nephrin Antibody: CNF NPHN nephrin Nephrin Renal glomerulus-specific cell adhesion receptor |
Application
Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 10 μg/mL.
Application
Immunohistochemistry of Nephrin in rat kidney tissue with Nephrin antibody at 10 μg/mL.
Application
Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 5 μg/mL.
Application
Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC-p:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nephrin Antibody is supplied in PBS containing 0.02% sodium azide. |
