Application
Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 μg/ml.Application
Immunofluorescence of Nephrin in human kidney tissue with Nephrin antibody at 20 μg/ml.Application
Western blot analysis of Nephrin in human kidney tissue lysate with Nephrin antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | Nephrin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3 |
| Product description | Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).1) Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein – Nephrin – is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582. |
| Immunogen | Nephrin antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Nephrin. The immunogen is located within amino acids 1100 - 1150 of Nephrin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 5 μg/ml. For immunofluorescence start at 20 μg/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPHS1 |
|---|---|
| Alternative Names | NPHN NPHS1 Renal glomerulus-specific cell adhesion receptor3 |
| Molecular Weight(MW) | Predicted: 125, 137 kDa Observed: 125 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | Nephrin antibody is human specific. |
Application
Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 μg/ml.
Application
Immunofluorescence of Nephrin in human kidney tissue with Nephrin antibody at 20 μg/ml.
Application
Western blot analysis of Nephrin in human kidney tissue lysate with Nephrin antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 5 μg/ml. For immunofluorescence start at 20 μg/mL: |
| Form | Liquid |
|---|---|
| Storage Instructions | Nephrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Nephrin antibody is supplied in PBS containing 0.02% sodium azide. |
