Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Product Name | NFIL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFIL3, E4BP4, IL3BP1, NFIL3A, NF-IL3A |
| Product description | NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia.1) Ozkurt, I.C., et al., (2003) J. Biol. Chem. 278 (29), 26803-26809. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIL3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFIL3 antibody can be used for detection of NFIL3 by ELISA at 1:312500. NFIL3 antibody can be used for detection of NFIL3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NFIL3 |
|---|---|
| Alternative Names | NFIL3 E4BP4 IL3BP1 NFIL3A NF-IL3A |
| Molecular Weight(MW) | 51 kDa |
Application
Antibody used in WB on Transfected 293T at 1.25 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | NFIL3 antibody can be used for detection of NFIL3 by ELISA at 1:312500. NFIL3 antibody can be used for detection of NFIL3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFIL3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
