WB
Various lysates were subjected to SDS PAGE followed by western blot with NFIL3 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using NFIL3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using NFIL3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using NFIL3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIL3 antibody at dilution of 1:800 and Omnimabs®594-Conjugated Goat Anti-Rabbit IgG(H+L) , CL488-phalloidin (green).IP
IP result of anti-NFIL3 (IP:4ug; Detection:1:1000) with HeLa cells lysate 1600ug.FC
1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | NFIL3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NFIL3 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1000-1:6000 IHC:1:500-1:2000 ICC/IF:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFIL3 |
|---|---|
| Gene Synonyms | E4BP4 IL3BP1 NFIL3A NF-IL3A |
| Gene Full Name | nuclear factor, interleukin 3 regulated |
| Gene Summary | The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014] |
| Alternative Names | NFIL3A Interleukin-3-binding protein 1 Interleukin-3 promoter transcriptional activator IL3BP1 E4BP4 |
| Molecular Weight(MW) | 51kDa(Observed MW:55-60kDa) |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with NFIL3 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using NFIL3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using NFIL3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using NFIL3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIL3 antibody at dilution of 1:800 and Omnimabs®594-Conjugated Goat Anti-Rabbit IgG(H+L) , CL488-phalloidin (green).
IP
IP result of anti-NFIL3 (IP:4ug; Detection:1:1000) with HeLa cells lysate 1600ug.
FC
1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:1000-1:6000 IHC:1:500-1:2000 ICC/IF:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
