NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.1) Yoo, Y.G., , et al., (2004) J. Biol. Chem. 279 (51), 53365-53373.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR4A1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NR4A1 antibody can be used for detection of NR4A1 by ELISA at 1:312500. NR4A1 antibody can be used for detection of NR4A1 by western blot at 2.0/mL.:
Antibody used in IHC on Human Stomach at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
NR4A1 antibody can be used for detection of NR4A1 by ELISA at 1:312500. NR4A1 antibody can be used for detection of NR4A1 by western blot at 2.0/mL.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR4A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.