NR4A1 encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.1) Lu, L., et al., (2004) J. Clin. Endocrinol. Metab. 89 (8), 4113-4118.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR4A1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NR4A1 antibody can be used for detection of NR4A1 by ELISA at 1:312500. NR4A1 antibody can be used for detection of NR4A1 by western blot at 1.45 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Testis.
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Stomach at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
NR4A1 antibody can be used for detection of NR4A1 by ELISA at 1:312500. NR4A1 antibody can be used for detection of NR4A1 by western blot at 1.45 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR4A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt