Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in WB on Human Hela at 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IF on Human HeLa.| Product Name | NUP50 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUP50, MGC39961, NPAP60, NPAP60L |
| Product description | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUP50. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NUP50 antibody can be used for detection of NUP50 by ELISA at 1:312500. NUP50 antibody can be used for detection of NUP50 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP50 |
|---|---|
| Alternative Names | NUP50 MGC39961 NPAP60 NPAP60L |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in WB on Human Hela at 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IF on Human HeLa.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NUP50 antibody can be used for detection of NUP50 by ELISA at 1:312500. NUP50 antibody can be used for detection of NUP50 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NUP50 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
