Western blot
Host: Rabbit Target Name: NUP50 Sample Tissue: Hela Antibody Dilution: 1.0ug/mlImmunofluorescence
Hela cell lysateWestern blot
Host: Rabbit Target Name: NUP50 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: NUP50 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-NUP50 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC39961, NPAP60, NPAP60L |
| Immunogen | The immunogen for anti-NUP50 antibody: synthetic peptide directed towards the C terminal of human NUP50 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500~1:2000 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP50 |
|---|---|
| Gene Synonyms | MGC39961 NPAP60 NPAP60L |
| Gene Full Name | Nucleoporin 50kDa |
| Gene Summary | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | MGC39961 NPAP60 NPAP60L |
| Molecular Weight(MW) | 50kDa |
| Sequence | 468 amino acids |
Western blot
Host: Rabbit Target Name: NUP50 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml
Immunofluorescence
Hela cell lysate
Western blot
Host: Rabbit Target Name: NUP50 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: NUP50 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
