Application
Western blot analysis of anti-NUP50 Pab in mouse brain tissue lysate.Application
Western blot analysis of NUP50 using rabbit polyclonal NUP50 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2).| Product Name | NUP50 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L |
| Product description | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.1) Lindsay, M.E., et al., Cell 110(3):349-360 (2002). |
| Immunogen | This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human NUP50. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | NUP50 |
|---|---|
| Alternative Names | Nuclear pore complex protein Nup50 50 kDa nucleoporin Nuclear pore-associated protein 60 kDa-like Nucleoporin Nup50 NUP50 NPAP60L |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of anti-NUP50 Pab in mouse brain tissue lysate.
Application
Western blot analysis of NUP50 using rabbit polyclonal NUP50 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
