Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PAK1 (phospho Ser204) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADRB2, PAK 1, P65-PAK, P68-PAK |
| Product description | The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.1) Brown J.L.,Curr. Biol. 6:598-605(1996). |
| Immunogen | PAK1 (phospho Ser204) antibody was raised against a peptide sequence around phosphorylation site of Serine 204 (T-R-S (p)-V-I) derived from Human PAK1. |
| Modification | p-Ser204 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK1 |
|---|---|
| Alternative Names | ADRB2 PAK 1 P65-PAK P68-PAK |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PAK1 only when phosphorylated at serine 204. |
| Entrez Gene | 5058 |
|---|---|
| Protein Accession | NP_002567.3 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
