Application
Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 μg/mL.Application
Immunohistochemistry of PD-1 in mouse thymus tissue with PD-1 antibody at 5 μg/mL.Application
Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 μg/mL.| Product Name | PD-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PD-1 Antibody [12A7D7] : PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1 |
| Product description | PD-1 Monoclonal Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. |
| Immunogen | A ~150 amino acid recombinant protein from near the amino terminus of mouse PD-1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDCD1 |
|---|---|
| Alternative Names | PD-1 Antibody [12A7D7] : PD1 PD-1 CD279 SLEB2 hPD-1 hPD-l hSLE1 |
Application
Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 μg/mL.
Application
Immunohistochemistry of PD-1 in mouse thymus tissue with PD-1 antibody at 5 μg/mL.
Application
Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 95-115 - PD-1 Recombinant Protein |
|---|---|
| Application Notes | PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PD-1 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | PD-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
