Application
Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [4C7] antibody at 1 μg/ml. Yellow: untransfected cells, PD-1 transfected cells.Application
Immunofluorescence of PD-1 in human breast cancer tissue with PD-1 [4C7] antibody at 10 μg/mL.Application
(A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [4C7] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.Application
Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [4C7] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.| Product Name | PD-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1 |
| Product description | Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. |
| Immunogen | PD-1 antibody was raised against the extracellular domain of human PD-1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICCIFIHC-PWB |
| PD-1 antibody can be used for detection of PD-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Flow cytometry at 0.1 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDCD1 |
|---|---|
| Alternative Names | PD-1 Antibody: PD1 PD-1 CD279 SLEB2 hPD-1 hPD-l hSLE1 PD1 Programmed cell death protein 1 Protein PD-1 PDCD1 PDCD-1 |
| Molecular Weight(MW) | Predicted: 32 kDa Observed: 38 kDa |
| Sequence Similarities | 4) Cat. No. 1208 - THP-1 Cell Lysate |
Application
Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [4C7] antibody at 1 μg/ml. Yellow: untransfected cells, PD-1 transfected cells.
Application
Immunofluorescence of PD-1 in human breast cancer tissue with PD-1 [4C7] antibody at 10 μg/mL.
Application
(A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [4C7] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.
Application
Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [4C7] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | PD-1 antibody can be used for detection of PD-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Flow cytometry at 0.1 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PD-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PD-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
