Application
Western blot analysis of lysed extracts from 293, HeLa and C6 cells using PKR (Ab-446).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR (Ab-446). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKR (Ab-446).| Product Name | PKR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKR, PRKR, EIF2AK1, APT1, FAS1, TNFRSF6, ADRB2, E2AK2, EIF2AK2, EIF2aK |
| Product description | Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.1) Abujiang Pataer, et, al. (2002) Cancer Res ; 62: 2239. K. D. Ryman, et, al. (2005) J. Virol ; 79: 1487 - 1499. Susana Guerra, et, al. (2006) J. Biol. Chem ; 281: 18734 - 18745. |
| Immunogen | PKR (Ab-446) antibody was raised against a peptide sequence around aa.444~448 (K-R-T-R-S) derived from Human PKR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK2 |
|---|---|
| Alternative Names | PKR PRKR EIF2AK1 APT1 FAS1 TNFRSF6 ADRB2 E2AK2 EIF2AK2 EIF2aK |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PKR protein. |
| Entrez Gene | 5610 |
|---|---|
| Protein Accession | NP_001129123.1 |
Application
Western blot analysis of lysed extracts from 293, HeLa and C6 cells using PKR (Ab-446).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR (Ab-446). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKR (Ab-446).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
