Application
Immunofluorescence of PKR in mouse lung tissue with PKR antibody at 20 μg/ml.Application
Immunohistochemistry of PKR in mouse lung tissue with PKR antibody at 5 μg/mL.Application
Immunohistochemistry of PKR in rat lung tissue with PKR antibody at 2.5 μg/mL.Application
Immunofluorescence of PKR in mouse lung tissue with PKR antibody at 20 μg/ml.Application
Western blot analysis of PKR in A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | PKR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2 |
| Product description | PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.1) Meurs E, Chong K, Galabru J, et al. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon. Cell 1990; 62:379-90. |
| Immunogen | PKR antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PKR antibody can be used for detection of PKR by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK2 |
|---|---|
| Alternative Names | PKR Antibody: PKR PRKR EIF2AK1 PKR Interferon-induced double-stranded RNA-activated protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 eIF-2A protein kinase 2 |
| Molecular Weight(MW) | Predicted: 56, 61 kDa Observed: 72 kDa |
| Sequence Similarities | 2) Cat. No. 1462 - Rat Lung Tissue Lysate |
| Tissue Specificity | At least two different isoforms of PKR are known to exist; this antibody will detect both isoforms. |
Application
Immunofluorescence of PKR in mouse lung tissue with PKR antibody at 20 μg/ml.
Application
Immunohistochemistry of PKR in mouse lung tissue with PKR antibody at 5 μg/mL.
Application
Immunohistochemistry of PKR in rat lung tissue with PKR antibody at 2.5 μg/mL.
Application
Immunofluorescence of PKR in mouse lung tissue with PKR antibody at 20 μg/ml.
Application
Western blot analysis of PKR in A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | PKR antibody can be used for detection of PKR by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PKR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PKR Antibody is supplied in PBS containing 0.02% sodium azide. |
