Application
Top Image: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR (Phospho-Thr446). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKR(Phospho-Thr446).Application
Western blot analysis of lysed extracts from K562 cells using PKR (Phospho-Thr446).| Product Name | PKR (phospho Thr446) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKR, PRKR, EIF2AK1, C1orf24, NIBAN, GIG39, ADRB2, E2AK2, EIF2AK2, EIF2aK |
| Product description | Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.1) Abujiang Pataer, et, al. (2002) Cancer Res; 62: 2239. K. D. Ryman, et, al. (2005) J. Virol; 79: 1487 - 1499. Susana Guerra, et, al. (2006) J. Biol. Chem; 281: 18734 - 18745. |
| Immunogen | PKR (Phospho-Thr446) antibody was raised against a peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR. |
| Modification | p-Thr446 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK2 |
|---|---|
| Alternative Names | PKR PRKR EIF2AK1 C1orf24 NIBAN GIG39 ADRB2 E2AK2 EIF2AK2 EIF2aK |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of PKR only when phosphorylated at threonine 446. |
| Entrez Gene | 5610 |
|---|---|
| Protein Accession | NP_001129123.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR (Phospho-Thr446). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKR(Phospho-Thr446).
Application
Western blot analysis of lysed extracts from K562 cells using PKR (Phospho-Thr446).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
