Application
Western blot analysis of PKR in MCF7 cell lysate with PKR antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of PKR in rat lung tissue with PKR antibody at 5 μg/mL.| Product Name | PKR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2 |
| Product description | PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.1) Meurs E, Chong K, Galabru J, et al. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon. Cell 1990; 62:379-90. |
| Immunogen | PKR antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PKR. The immunogen is located within the first 50 amino acids of PKR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| PKR antibody can be used for detection of PKR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK2 |
|---|---|
| Alternative Names | PKR Antibody: PKR PRKR EIF2AK1 PKR Interferon-induced double-stranded RNA-activated protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 eIF-2A protein kinase 2 |
| Molecular Weight(MW) | Predicted: 61 kDa Observed: 72 kDa |
| Sequence Similarities | 2) Cat. No. 1462 - Rat Lung Tissue Lysate |
Application
Western blot analysis of PKR in MCF7 cell lysate with PKR antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of PKR in rat lung tissue with PKR antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | PKR antibody can be used for detection of PKR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PKR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PKR Antibody is supplied in PBS containing 0.02% sodium azide. |
