Application
Immunohistochemistry staining of PRMT5 in testis tissue using PRMT5 Antibody.| Product Name | PRMT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT5, 72 kDa ICln-binding protein, HRMT1L5, JBP1, Jak-binding protein 1, IBP72, SKB1Hs, SKB1, SKB1 homolog, Skb1 (S. pombe) homolog, SKB1 homolog (S. pombe) |
| Product description | Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro. Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast twohybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK). Both proteins belong to the protein methyltransferase superfamily. Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells. Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin sections), and Immunocytochemistry (ABC). Other applications have not been tested. Detects endogenous levels of total human Skb1Hs methyltransferase protein. Does not crossreact with other related mammalian methyltransferases. |
| Immunogen | PRMT5 antibody was raised against a synthetic peptide corresponding to residues surrounding amino acid 100 of PRMT5 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| PRMT5 antibody can be used in Western Blot starting at 1 μg/mL, and immunohistochemistry starting at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRMT5 |
|---|---|
| Alternative Names | PRMT5 72 kDa ICln-binding protein HRMT1L5 JBP1 Jak-binding protein 1 IBP72 SKB1Hs SKB1 SKB1 homolog Skb1 (S. pombe) homolog SKB1 homolog (S. pombe) |
| Tissue Specificity | Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not cross-react with other related mammalian methyltransferases. |
Application
Immunohistochemistry staining of PRMT5 in testis tissue using PRMT5 Antibody.| Application Notes | PRMT5 antibody can be used in Western Blot starting at 1 μg/mL, and immunohistochemistry starting at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store PRMT5 antibody should be stored at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50% glycerol. No preservative added. |
